{"id":1829,"date":"2022-09-13T08:16:56","date_gmt":"2022-09-13T00:16:56","guid":{"rendered":"http:\/\/www.sunlonge.cn\/?p=1829"},"modified":"2022-09-13T08:18:07","modified_gmt":"2022-09-13T00:18:07","slug":"gfp%e6%bf%80%e5%8f%91%e8%a7%82%e6%b5%8b%e5%85%89%e6%ba%90%e5%9c%a8%e7%a7%91%e5%ad%a6%e7%a0%94%e7%a9%b6%e7%9a%84%e5%ba%94%e7%94%a8","status":"publish","type":"post","link":"https:\/\/www.sunlonge.cn\/1829.html","title":{"rendered":"GFP+\u6fc0\u53d1\u89c2\u6d4b\u5149\u6e90\u5728\u79d1\u5b66\u7814\u7a76\u7684\u5e94\u7528"},"content":{"rendered":"<p><a target=\"_blank\" href=\"http:\/\/www.sunlonge.cn\/products\/swjfgcgy\">\u6fc0\u53d1\u5149\u6e90<\/a>\u6fc0\u53d1GFP\uff08\u7eff\u8272\u8367\u5149\u86cb\u767dGreen Fluorescent 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\/>\n\u7eff\u8272\u8367\u5149\u86cb\u767d\u8d28\u7c92\u8f6c\u67d3\u5177\u6709\u64cd\u4f5c\u7b80\u4fbf\u3001\u5bf9\u5e72\u7ec6\u80de\u7684\u514d\u75ab\u6e90\u6027\u548c\u6bd2\u6027\u5c0f\u7b49\u4f18\u70b9\uff0c\u4f46\u4e0d\u5341\u5206\u7a33\u5b9a\uff0c\u5bb9\u6613\u4e22\u5931\uff1b<br \/>\n\u75c5\u6bd2\u8f7d\u4f53\u7684\u4f18\u52bf\u662f\u8868\u8fbe\u9ad8\u6548\u7a33\u5b9a\uff0c\u4f46\u5b58\u5728\u514d\u75ab\u53cd\u5e94\u548c\u81f4\u764c\u6027\u7b49\u5b89\u5168\u9690\u60a3\uff1b<br \/>\n\u4ece\u7eff\u8272\u8367\u5149\u86cb\u767d\u8f6c\u57fa\u56e0\u52a8\u7269\u5206\u79bb\u5f97\u5230\u7684\u5e72\u7ec6\u80de\u6807\u8bb0\u7a33\u5b9a\uff0c\u662f\u7406\u60f3\u7684\u9009\u62e9\u3002\u7eff\u8272\u8367\u5149\u86cb\u767d\u6807\u8bb0\u7684\u5e72\u7ec6\u80de\u79fb\u690d\u540e\u5728\u4f53\u5185\u7684\u5206\u5e03\u60c5\u51b5\u53ef\u5728\u8367\u5149\u663e\u5fae\u955c\u4e0b\u76f4\u63a5\u89c2\u5bdf\u5230\uff0c\u7ed3\u5408\u4f7f\u7528\u5b9a\u91cf\u805a\u5408\u9176\u94fe\u53cd\u5e94\u3001\u6fc0\u5149\u5171\u805a\u7126\u548c\u8367\u5149\u6fc0\u6d3b\u7ec6\u80de\u5206\u7c7b\u8ba1\u6570\u7b49\u6280\u672f\u65b9\u6cd5\uff0c\u8fd8\u53ef\u76d1\u6d4b\u79fb\u690d\u5e72\u7ec6\u80de\u5728\u4f53\u5185\u7684\u5206\u5316\u589e\u751f\u60c5\u51b5\u4ee5\u53ca\u5176\u5728\u5404\u7ec4\u7ec7\u7684\u542b\u91cf\u7b49\u3002<\/p>\n<p>&#8211;\u5728\u7ec6\u80de\u751f\u7269\u5b66\u4e2d\u7684\u5e94\u7528<\/p>\n<p>GFP\u5177\u6709\u540c\u5bbf\u4e3b\u86cb\u767d\u6784\u6210\u878d\u5408\u5b50\u7684\u6027\u8d28\uff0c\u5229\u7528\u8fd9\u4e00\u6027\u8d28\uff0c\u53ef\u4ee5\u5c06GFP\u5b9a\u4f4d\u5230\u7279\u5b9a\u7684\u7ec6\u80de\u5668\u548c\u819c\u7cfb\u7edf\u4e2d\uff0c\u8fdb\u884c\u7ec6\u80de\u751f\u7406\u8fc7\u7a0b\u3001\u7ec6\u80de\u52a8\u529b\u5b66\u7b49\u7684\u5b9e\u65f6\u89c2\u6d4b\uff0c\u6216\u76f4\u63a5\u5e94\u7528\u4e8e\u5b9a\u91cf\u5206\u6790\u3002\u76ee\u524d\uff0cGFP\u5df2\u7ecf\u88ab\u6210\u529f\u5730\u7528\u4e8e\u9776\u5411\u6807\u8bb0\u5305\u62ec\u7ec6\u80de\u6838\u3001\u7ebf\u7c92\u4f53\u3001\u8d28\u4f53\u3001\u5185\u8d28\u7f51\u7b49\u5728\u5185\u7684\u7ec6\u80de\u5668\u3002\u7528GFP\u8fdb\u884c\u4e9a\u7ec6\u80de\u5b9a\u4f4d\uff0c\u907f\u514d\u4e86\u63d0\u7eaf\u86cb\u767d\u3001\u6807\u8bb0\u5f02\u786b\u6c30\u9178\u8367\u5149\u7d20\u7b49\u8367\u5149\u67d3\u6599\u3001\u7ecf\u663e\u5fae\u6ce8\u5c04\u6216\u5176\u4ed6\u65b9\u5f0f\u5bfc\u5165\u7ec6\u80de\u7684\u590d\u6742\u65b9\u6cd5\uff0c\u4ece\u800c\u4f7f\u7814\u7a76\u86cb\u767d\u5728\u6d3b\u7ec6\u80de\u7684\u51c6\u786e\u5b9a\u4f4d\u53d8\u5f97\u7b80\u5355\u6613\u884c\u3002<\/p>\n<p>&#8211;\u5728\u5206\u5b50\u751f\u7269\u5b66\u7814\u7a76\u4e2d\u7684\u5e94\u7528<\/p>\n<p>&#8211;\u5728\u751f\u7269\u5b66\u7814\u7a76\u4e2d\u7684\u5e94\u7528<br \/>\nGFP\u4f5c\u4e3a\u6807\u8bb0\u5206\u5b50\uff0c\u76ee\u524d\u5df2\u88ab\u5e7f\u6cdb\u5e94\u7528\u4e8e\u57fa\u56e0\u6807\u8bb0\u3001\u86cb\u767d\u8d28\u6807\u8bb0\u3001\u73af\u5883\u5fae\u751f\u7269\u7814\u7a76\u3001\u5bc4\u751f\u866b\u5b66\u7814\u7a76\u4ee5\u53ca\u53d1\u80b2\u751f\u7269\u5b66\u7814\u7a76\u4e2d\u57fa\u56e0\u8868\u8fbe\u6a21\u5f0f\u7684\u63a2\u7d22\u7b49\u65b9\u9762\uff0c\u6210\u4e3a\u6d3b\u7ec6\u80de\u5206\u5b50\u6c34\u5e73\u7814\u7a76\u7684\u5de5\u5177\u3002<\/p>\n<p>&#8211;\u5728\u751f\u6001\u5b66\u4e0a\u7684\u5e94\u7528<br \/>\nGFP\u5728\u751f\u6001\u5b66\u4e2d\u7684\u5e94\u7528\u5c06GFP\u57fa\u56e0\u76f4\u63a5\u5bfc\u5165\u76ee\u6807\u751f\u7269\u6216\u5c06GFP\u57fa\u56e0\u514b\u9686\u5230\u75c5\u6bd2\u3001\u7ec6\u83cc\u6216\u8d28\u7c92\u4e0a\uff0c\u901a\u8fc7\u5b83\u4eec\u7684\u4ecb\u5bfc\u800c\u95f4\u63a5\u6807\u8bb0\u76ee\u6807\u751f\u7269\uff0c\u8fdb\u884c\u751f\u6001\u5b66\u89c4\u5f8b\u7814\u7a76\u3002<br \/>\n\u7528GFP\u57fa\u56e0\u6807\u8bb0\u9057\u4f20\u5de5\u7a0b\u5fae\u751f\u7269\u4ee5\u68c0\u6d4b\u5176\u5728\u6c34\u73af\u5883\u4e2d\u7684\u5b58\u6d3b\u548c\u53bb\u5411\u3002<br \/>\n\u8ddf\u8e2a\u89c2\u5bdf\u5fae\u751f\u7269\u3002<\/p>\n<p>&#8211;\u5728\u533b\u5b66\u7814\u7a76\u4e2d\u7684\u5e94\u7528<\/p>\n<p>\u5229\u7528GFP\u793a\u8e2a\u8bc1\u5b9e\u4e86\u8102\u80aa\u6765\u6e90\u7684\u5e72\u7ec6\u80de\u7684\u5206\u5316\u65b9\u5411\u548c\u5206\u5316\u80fd\u529b\uff1b\u5229\u7528\u589e\u5f3a\u578bGFP\u7b5b\u9009\u5408\u9002\u7684\u751f\u7269\u6750\u6599\uff1b GFP\u8fd8\u7528\u4e8e\u7ec6\u80de\u56e0\u5b50\u4f5c\u7528\u7684\u7814\u7a76\u4ee5\u53ca\u6d3b\u4f53\u52a8\u6001\u89c2\u5bdf\u7684\u7814\u7a76\u3002<br \/>\nGFP\u5e7f\u6cdb\u5e94\u7528\u4e8e\u89c6\u7f51\u819c\u7684\u57fa\u56e0\u8f6c\u79fb\u7cfb\u7edf\u3001\u76ee\u7684\u57fa\u56e0\u7684\u8c03\u63a7\u53ca\u611f\u5149\u7ec6\u80de\u51cb\u4ea1\u4fdd\u62a4\u7684\u7814\u7a76\u3002<br \/>\n\u5728\u514d\u75ab\u5b66\u6cbb\u7597\u65b9\u9762\uff0c\u5e94\u7528GFP\u4f5c\u4e3a\u8868\u76ae\u751f\u957f\u56e0\u5b50\u53d7\u4f53\u7814\u7a76\u7684\u5de5\u5177\uff0c\u5e76\u5bf9\u7b2c\u4e8c\u4fe1\u4f7f\u86cb\u767d\u8fdb\u884c\u4e9a\u7ec6\u80de\u5b9a\u4f4d\uff0c\u8fd9\u65e2\u53ef\u4ee5\u63ed\u793a\u5176\u5728\u6d3b\u7ec6\u80de\u5185\u4f5c\u7528\uff0c\u53c8\u53ef\u4ee5\u89c2\u5bdf\u4e00\u4e9b\u6297\u4f53\u548c\u5c0f\u5206\u5b50\u916a\u6c28\u9178\u6fc0\u9176\u6291\u5236\u5242\u7b49\u4fe1\u53f7\u4f20\u5bfc\u6291\u5236\u836f\u7269\u7684\u4f5c\u7528\u6548\u5e94\u3002<\/p>\n<p>&#8211;\u4f5c\u4e3a\u62a5\u544a\u57fa\u56e0<\/p>\n<p>GFP\u4f5c\u4e3a\u62a5\u544a\u57fa\u56e0\u53ef\u7528\u6765\u68c0\u6d4b\u8f6c\u57fa\u56e0\u6548\u7387\uff0c\u628aGFP\u57fa\u56e0\u8fde\u63a5\u5230\u76ee\u7684\u57fa\u56e0\u7684\u542f\u52a8\u5b50\u4e4b\u540e\uff0c\u901a\u8fc7\u6d4b\u5b9aGFP\u7684\u8367\u5149\u5f3a\u5ea6\u5c31\u53ef\u4ee5\u5bf9\u8be5\u57fa\u56e0\u7684\u8868\u8fbe\u6c34\u5e73\u8fdb\u884c\u68c0\u6d4b\u3002\u76ee\u524d\uff0c\u6b64\u65b9\u6cd5\u65e0\u8bba\u5728\u519c\u6746\u83cc\u4ecb\u5bfc\u6216\u57fa\u56e0\u67aa\u4ecb\u5bfc\u7684\u690d\u7269\u9057\u4f20\u8f6c\u5316\u4e2d\u8fd8\u662f\u5728\u6d3b\u7ec6\u80de\u3001\u8f6c\u57fa\u56e0\u80da\u80ce\u548c\u52a8\u7269\u4e2d\u90fd\u5df2\u5f97\u5230\u975e\u5e38\u5e7f\u6cdb\u7684\u5e94\u7528\uff0c\u7279\u522b\u662f\u5728\u6d3b\u7ec6\u80de\u57fa\u56e0\u8868\u8fbe\u7684\u65f6\u7a7a\u6210\u50cf\u65b9\u9762\u3002<\/p>\n<p>&#8211;\u4f5c\u4e3a\u878d\u5408\u6807\u7b7e<\/p>\n<p>GFP\u6700\u6210\u529f\u7684\u4e00\u7c7b\u5e94\u7528\u5c31\u662f\u628aGFP\u4f5c\u4e3a\u6807\u7b7e\u878d\u5408\u5230\u4e3b\u4f53\u86cb\u767d\u4e2d\u6765\u68c0\u6d4b\u86cb\u767d\u8d28\u5206\u5b50\u7684\u5b9a\u4f4d\u3001\u8fc1\u79fb\u3001\u6784\u8c61\u53d8\u5316\u4ee5\u53ca\u5206\u5b50\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\uff0c\u6216\u8005\u9776\u5411\u6807\u8bb0\u67d0\u4e9b\u7ec6\u80de\u5668\u3002\u5728\u591a\u6570\u60c5\u51b5\u4e0b\uff0cGFP\u57fa\u56e0\u5728N-\u6216C-\u672b\u7aef\u4e0e\u5f02\u6e90\u57fa\u56e0\u7528\u5e38\u89c4\u7684\u5206\u5b50\u751f\u7269\u5b66\u624b\u6bb5\u5c31\u53ef\u4ee5\u7ed3\u5408\u6784\u6210\u7f16\u7801\u878d\u5408\u86cb\u767d\u7684\u5d4c\u5408\u57fa\u56e0\uff0c\u5176\u8868\u8fbe\u4ea7\u7269\u65e2\u4fdd\u6301\u4e86\u5916\u6e90\u86cb\u767d\u7684\u751f\u7269\u6d3b\u6027\uff0c\u53c8\u8868\u73b0\u51fa\u4e0e\u5929\u7136GFP\u76f8\u4f3c\u7684\u8367\u5149\u7279\u6027\u3002GFP\u7684\u8fd9\u79cd\u7279\u6027\u4e3a\u86cb\u767d\u8d28\u63d0\u4f9b\u4e86\u4e00\u79cd\u8367\u5149\u6807\u8bb0\uff0c\u4e0d\u4ec5\u53ef\u4ee5\u68c0\u6d4b\u86cb\u767d\u8d28\u5206\u5b50\u7684\u5b9a\u4f4d\u3001\u8fc1\u79fb\uff0c\u8fd8\u53ef\u4ee5\u7814\u7a76\u86cb\u767d\u8d28\u5206\u5b50\u7684\u76f8\u4e92\u4f5c\u7528\u4ee5\u53ca\u86cb\u767d\u8d28\u6784\u8c61\u53d8\u5316\uff0c\u5e76\u4f9d\u9760\u8367\u5149\u5171\u632f\u80fd\u91cf\u8f6c\u79fb\u6765\u8fdb\u884c\u68c0\u6d4b\u3002<\/p>\n<p>&#8211;\u5176\u4ed6\u5e94\u7528<\/p>\n<p>\u91ce\u751f\u578bGFP\u548c\u5176\u8bb8\u591a\u7a81\u53d8\u4f53\u53ef\u4ee5\u4f5c\u4e3a\u751f\u7269\u4f20\u611f\u5668\u68c0\u6d4b\u6d3b\u7ec6\u80de\u5185\u7684pH\u3002<br \/>\nGFP\u8fd8\u53ef\u4ee5\u5e94\u7528\u4e8e\u68c0\u6d4b\u7535\u4f4d\u3001\u6c27\u5316\u8fd8\u539f\u6c34\u5e73\u4ee5\u53ca\u5728\u4fe1\u53f7\u8f6c\u5bfc\u4e2d\u4f5c\u4e3aCa2+\u6307\u793a\u5242\u3002<br \/>\nGFP\u5e7f\u6cdb\u5e94\u7528\u4e8e\u7ec6\u80de\u7b5b\u9009\u548c\u836f\u7269\u7b5b\u9009 \u3002<br \/>\n\u901a\u8fc7\u5b9a\u70b9\u7a81\u53d8\u3001DNA-shuffling\u2002\u7b49\u6280\u672f\u5bf9GFP\u8fdb\u884c\u4e86\u6539\u8fdb\uff0c\u83b7\u5f97\u4e86\u8367\u5149\u5149\u8c31\u3001\u91cf\u5b50\u4ea7\u7387\u3001\u6eb6\u89e3\u6027\u3001\u5bc6\u7801\u5b50\u55dc\u6027\u3001\u6e29\u5ea6\u654f\u611f\u6027\u7b49\u6539\u53d8\u7684\u591a\u79cd\u7a81\u53d8\u4f53\uff0c\u6269\u5927\u4e86GFP\u7684\u5e94\u7528\u8303\u56f4\u3002<\/p>\n<p>\u6df1\u5733\u8367\u9e3f\u4e13\u4e1a\u7814\u53d1\u751f\u4ea7\u5404\u79cd<a target=\"_blank\" 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