{"id":1370,"date":"2020-11-30T08:38:20","date_gmt":"2020-11-30T00:38:20","guid":{"rendered":"http:\/\/www.sunlonge.cn\/?p=1370"},"modified":"2020-11-30T08:38:20","modified_gmt":"2020-11-30T00:38:20","slug":"%e8%8d%a7%e5%85%89%e8%9b%8b%e7%99%bd%e6%bf%80%e5%8f%91%e7%9a%84%e6%97%a5%e5%b8%b8%e7%94%a8%e9%80%94","status":"publish","type":"post","link":"https:\/\/www.sunlonge.cn\/1370.html","title":{"rendered":"\u8367\u5149\u86cb\u767d\u6fc0\u53d1\u7684\u65e5\u5e38\u7528\u9014"},"content":{"rendered":"<p>\n\t\u8367\u5149\u86cb\u767d+<a target=\"_blank\" href=\"http:\/\/www.sunlonge.cn\/products\/jifagyuan\">\u6fc0\u53d1\u5149\u6e90<\/a>\u5728\u751f\u7269\u57fa\u56e0\u7814\u7a76\u4e2d\u53d1\u6325\u7740\u5de8\u5927\u7684\u4f5c\u7528\uff0c\u8367\u5149\u86cb\u767d\u5728\u6fc0\u53d1\u5149\u6e90\u7684\u6fc0\u53d1\u4e0b\u53d1\u51fa\u8367\u5149\uff0c\u4f7f\u5f97\u7814\u7a76\u6548\u679c\u66f4\u76f4\u89c2\u3002\u4ee5\u4e0b\u4e3a\u8367\u5149\u86cb\u767d\u768410\u4e2a\u65e5\u5e38\u7528\u9014\uff0c\u672c\u6587\u4e3a\u6df1\u5733\u8367\u9e3f\u6458\u5f55\uff0c\u4ec5\u4f9b\u53c2\u8003\u3002\u5173\u4e8e\u6fc0\u53d1\u5149\u6e90\u9009\u62e9\uff0c\u8bf7\u54a8\u8be2\u6df1\u5733\u8367\u9e3f\uff1a0755-89233889.\n<\/p>\n<p>\n\t\uff081\uff09 Localization\uff1a\u53ef\u4ee5\u653e\u5728\u76ee\u7684\u57fa\u56e0\u7684N\u7aef\uff0c\u4e5f\u53ef\u4ee5\u653e\u5728\u76ee\u7684\u57fa\u56e0\u7684C\u7aef\u3002\u8fd9\u6837\u7684\u6784\u5efa\u65b9\u5f0f\u53ef\u4ee5\u5e2e\u52a9\u6211\u4eec\u89c2\u5bdf\u5230\u76ee\u7684\u57fa\u56e0\u662f\u4ec0\u4e48\u65f6\u5019\u5f00\u59cb\u8868\u8fbe\u4ee5\u53ca\u5728\u54ea\u91cc\u8868\u8fbe\uff1b\n<\/p>\n<p>\n\t\uff082\uff09 Transcription reporter\uff1a\u5c06\u8367\u5149\u86cb\u767d\u653e\u5728\u5f85\u7814\u7a76\u542f\u52a8\u5b50\u7684\u540e\u9762\uff0c\u53ef\u4ee5\u5f88\u597d\u7684\u89c2\u5bdf\u6216\u8005\u9a8c\u8bc1\u8be5\u542f\u52a8\u5b50\u5728\u7279\u5b9a\u7ec6\u80de\u4e2d\u7684\u542f\u52a8\u6d3b\u6027\uff1b\n<\/p>\n<p>\n\t\uff083\uff09 FRET(Frster Resonance Energy Transfer):\u7528\u6765\u7814\u7a76\u4e24\u4e2a\u4e0d\u540c\u7684\u86cb\u767d\u6216\u8005\u7528\u4e00\u4e2a\u86cb\u767d\u7684\u4e24\u4e2a\u4e0d\u540c\u7ed3\u6784\u57df\u4e4b\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u5173\u7cfb\u3002\u901a\u5e38\u662f\u4f7f\u7528\u6fc0\u53d1\/\u53d1\u5c04\u5149\u8c31\u6709\u91cd\u53e0\u7684\u4e24\u4e2a\u8367\u5149\u86cb\u767d\u3002\u76ee\u524d\u86cb\u767d-\u86cb\u767d\u76f8\u4e92\u4f5c\u7528\u7814\u7a76\u4e2d\u6700\u5e7f\u6cdb\u5e94\u7528\u7684FRET\u5bf9\u5c31\u662f\u9752\u8272\u8367\u5149\u86cb\u767d\uff08cyan fluorescent protein, CFP\uff09\u3001\u9ec4\u8272\u8367\u5149\u86cb\u767d\uff08yellow fluorescent protein, YFP\uff09\u3002\n<\/p>\n<p>\n\t\uff084\uff09 Split EGFP\uff1aFRET\u7684\u53e6\u4e00\u79cd\u5b9e\u73b0\u65b9\u6cd5\uff0c\u540c\u6837\u88ab\u7528\u6765\u7814\u7a76\u86cb\u767d\u4e0e\u86cb\u767d\u7684\u76f8\u4e92\u4f5c\u7528\u3002\u5177\u4f53\u662f\u5c06EGFP\u5206\u5272\u6210\u4e24\u90e8\u5206\uff0c\u5206\u522b\u878d\u5408\u5230\u4e24\u4e2a\u86cb\u767d\uff0c\u5f53\u4e24\u4e2a\u86cb\u767d\u9760\u8fd1\u65f6\uff0cEGFP\u7684\u4e24\u90e8\u5206\u86cb\u767d\u5f00\u59cb\u6298\u53e0\uff0c\u6210\u719f\u5230\u53d1\u5149\u3002\n<\/p>\n<p>\n\t\uff085\uff09 Biosensors\uff1a\u7528\u6765\u76d1\u6d4b\u7ec6\u80de\u5185\u5c0f\u751f\u7269\u5206\u5b50\u6216\u5176\u4ed6\u751f\u7406\u8fc7\u7a0b\uff0c\u6bd4\u5982AAV\u8f7d\u4f53\u4e2d\u7684\u9499\u6307\u793a\u5242\u3002\n<\/p>\n<p>\n\t\uff086\uff09 Optogenetics\uff1a\u662f\u7814\u7a76\u4eba\u5458\u4f7f\u7528\u4e00\u79cd\u65b0\u7684\u5149\u63a7\u65b9\u6cd5\u9009\u62e9\u5e76\u6253\u5f00\u4e86\u67d0\u79cd\u751f\u7269\u7684\u4e00\u7c7b\u7ec6\u80de\u3002\u5149\u9057\u4f20\u6280\u672f&#8211;21\u4e16\u7eaa\u795e\u7ecf\u79d1\u5b66\u9886\u57df\u6700\u5f15\u4eba\u6ce8\u76ee\u7684\u9769\u65b0\uff0c\u5176\u4e3b\u8981\u539f\u7406\u662f\u9996\u5148\u91c7\u7528\u57fa\u56e0\u64cd\u4f5c\u6280\u672f\u5c06\u5149\u611f\u57fa\u56e0\uff08\u5982ChR2\uff0ceBR\uff0cNaHR3.0\uff0cArch\u6216OptoXR\u7b49\uff09\u8f6c\u5165\u5230\u795e\u7ecf\u7cfb\u7edf\u4e2d\u7279\u5b9a\u7c7b\u578b\u7684\u7ec6\u80de\u4e2d\u8fdb\u884c\u7279\u6b8a\u79bb\u5b50\u901a\u9053\u6216GPCR\u7684\u8868\u8fbe\u3002\u5149\u611f\u79bb\u5b50\u901a\u9053\u5728\u4e0d\u540c\u6ce2\u957f\u7684\u5149\u7167\u523a\u6fc0\u4e0b\u4f1a\u5206\u522b\u5bf9\u9633\u79bb\u5b50\u6216\u8005\u9634\u79bb\u5b50\u7684\u901a\u8fc7\u4ea7\u751f\u9009\u62e9\u6027\uff0c\u4ece\u800c\u9020\u6210\u7ec6\u80de\u819c\u4e24\u8fb9\u7684\u819c\u7535\u4f4d\u53d1\u751f\u53d8\u5316\uff0c\u8fbe\u5230\u5bf9\u7ec6\u80de\u9009\u62e9\u6027\u5730\u5174\u594b\u6216\u8005\u6291\u5236\u7684\u76ee\u7684\u3002\n<\/p>\n<p>\n\t\uff087\uff09 Chemogenetics\uff1a\u5229\u7528\u9057\u4f20\u5b66\u539f\u7406\uff0c\u4ee5\u5316\u5b66\u5c0f\u5206\u5b50\u4e3a\u5de5\u5177\u89e3\u51b3\u751f\u7269\u7684\u95ee\u9898\uff0c\u6216\u901a\u8fc7\u5e72\u6270\u3001\u8c03\u8282\u6b63\u5e38\u751f\u7406\u8fc7\u7a0b\u4e86\u89e3\u86cb\u767d\u8d28\u7684\u529f\u80fd\u3002\n<\/p>\n<p>\n\t\uff088\uff09 In Vivo Imaging\uff1a\u6d3b\u4f53\u6210\u50cf\u7cfb\u7edf\u6210\u50cf\u539f\u7406\u5305\u62ec\u751f\u7269\u53d1\u5149\u4e0e\u8367\u5149\u4e24\u79cd\u6280\u672f\u3002\u751f\u7269\u53d1\u5149\u662f\u7528\u8367\u5149\u7d20\u9176\u57fa\u56e0\u6807\u8bb0DNA\uff0c\u5229\u7528\u5176\u4ea7\u751f\u7684\u86cb\u767d\u9176\u4e0e\u76f8\u5e94\u5e95\u7269\u53d1\u751f\u751f\u5316\u53cd\u5e94\u4ea7\u751f\u751f\u7269\u4f53\u5185\u7684\u5149\u4fe1\u53f7;\u800c\u8367\u5149\u6280\u672f\u5219\u91c7\u7528\u8367\u5149\u62a5\u544a\u57fa\u56e0(GFP\u3001RFP)\u6216\u8367\u5149\u67d3\u6599(\u5305\u62ec\u8367\u5149\u91cf\u5b50\u70b9)\u7b49\u65b0\u578b\u7eb3\u7c73\u6807\u8bb0\u6750\u6599\u8fdb\u884c\u6807\u8bb0\uff0c\u5229\u7528\u62a5\u544a\u57fa\u56e0\u4ea7\u751f\u7684\u751f\u7269\u53d1\u5149\u3001\u8367\u5149\u86cb\u767d\u8d28\u6216\u67d3\u6599\u4ea7\u751f\u7684\u8367\u5149\u5c31\u53ef\u4ee5\u5f62\u6210\u4f53\u5185\u7684\u751f\u7269\u5149\u6e90\u3002\u524d\u8005\u662f\u52a8\u7269\u4f53\u5185\u7684\u81ea\u53d1\u8367\u5149\uff0c\u4e0d\u9700\u8981\u6fc0\u53d1\u5149\u6e90\uff0c\u800c\u540e\u8005\u5219\u9700\u8981\u5916\u754c\u6fc0\u53d1\u5149\u6e90\u7684\u6fc0\u53d1\u3002\n<\/p>\n<p>\n\t\uff089\uff09 Cell marking\/selection\uff1a\u5927\u5bb6\u505a\u7ec6\u80de\u5b9e\u9a8c\uff0c\u901a\u5e38\u90fd\u559c\u6b22\u8d28\u7c92\u5e26\u6709\u8367\u5149\u6807\u7b7e\u6bd4\u5982GFP\uff0c\u6709\u4e86\u8be5\u8367\u5149\u6807\u7b7e\u53ef\u4ee5\u5f88\u65b9\u4fbf\u7684\u5224\u65ad\u8d28\u7c92\u7684\u8f6c\u67d3\u6548\u7387\u3002\u8fd9\u79cd\u60c5\u51b5\u901a\u5e38\u662f\u8367\u5149\u86cb\u767d\u7531\u53e6\u5916\u4e00\u4e2a\u4e0d\u540c\u7684\u542f\u52a8\u5b50\u63a7\u5236\u6216\u8005\u7531\u4e0e\u76ee\u7684\u57fa\u56e0\u76f8\u540c\u7684\u542f\u52a8\u5b50\u63a7\u5236\u4f46\u662f\u901a\u8fc7IRES\u8fde\u63a5\u3002\n<\/p>\n<p>\n\t\uff0810\uff09 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